Its autonomous Ford Fusions will have human back-up drivers but will transport passengers just like normal Uber vehicles, the company said.
Uber has a self-driving research lab in Pittsburgh and is working on autonomous technology.
Uber and Volvo also announced a 300 million dollar (£228 million) deal for Volvo to provide SUVs to Uber for autonomous vehicle research.
Eventually the Volvo SUVs will be part of the self-driving fleet in Pittsburgh.
Volvo will develop base vehicles for research and both companies will develop autonomous vehicles on their own.
The moves are intended to significantly accelerate Uber into the race to deploy self-driving vehicles to the public.
It is also the latest tie-up between Silicon Valley, ride-hailing firms and major carmakers.
Uber's chief executive Travis Kalanick, has said the ride-sharing company's future - and the future of all transportation - is driverless.