The incubation hub based at iCentrum has been open since May and is already home to 14 businesses across a range of sectors including property, media, sports nutrition and agriculture.
The Eagle Lab provides access to resources including mentoring, event space, 3D printers and laser cutters.
The lab aims to boost the productivity and success of local entrepreneurs, businesses and business communities.
Ray O’Donoghue, managing director for Barclays in the Midlands said: “Birmingham was recently crowned the ‘most enterprising place in Britain’ by the 2016 Enterprising Britain Awards and the initial interest we have had in the Eagle Lab is testimony to this entrepreneurial spirit.
“Whilst we have reached capacity and are seeing the really positive impact the site has had on our early stage businesses we will soon be starting the search for a new batch of entrepreneurs to support.”
Businesses sign up to work with the Eagle Lab for a period of six months and therefore Barclays will be recruiting new businesses an on-going basis.