The airline has launched five times weekly services to Prague and offers a strong network of connections through Eastern Europe.
The Airbus A319 aircraft, with business and economy cabins, was welcomed with a traditional water arch salute from the airport’s fire service on its arrival into Birmingham.
William Pearson, Birmingham Airport’s aviation development director, said: “We are delighted to welcome Czech Airlines as a new partner, serving Prague five times a week.
“This direct route, which was unserved from Birmingham Airport, gives passengers the chance to visit this picturesque city, known as The City of a Hundred Spires, for either business or leisure at very reasonable prices.”
The service could also provide a welcome boost to the regions exporters. UK exports to the Czech Republic reached £1.95bn in 2013, making the country the UK’s 29th largest export market.