The milestone will be achieved at Saturday's game between Chelsea and Everton, when all match-day staff and caterers will be on the voluntary rates - which are higher than the National Living Wage.
The new rates were announced earlier this week, at £9.75 an hour in London and £8.45 in the rest of the UK - compared with the statutory figure of £7.20 for adults.
Katherine Chapman, director of the Living Wage Foundation, said: "To watch the first ever Premier League Living Wage football match is the perfect way to mark the end of this year's Living Wage Week, where we celebrate almost 3,000 employers who have committed to pay all their staff enough to cover the cost of living.
"This is the first time two Premier League clubs paying the real Living Wage will meet on the pitch.
"I hope that this will inspire other football clubs to follow suit so we can have Living Wage matches every week."
Chelsea were the first Premier League side to become an accredited Living Wage club two years ago, followed by Everton last month.
The first ever Premier League match is to be played between two teams who both pay their workers the voluntary Living Wage.