The RGF initiative has made 792 investments in businesses, increasing overall turnover by £360m and creating and safeguarding more than 11,000 jobs.
James Newman, chairman of Finance Yorkshire, said the £100m milestone was a “tremendous achievement for everyone involved.”
He added: “The fund’s investments have made a significant impact on SMEs involved in a wide range of manufacturing and service sectors, in all parts of Yorkshire and the Humber.
“Many companies have found it difficult to access funding via traditional routes making our role invaluable.
“SMEs are the bedrock of our regional economy, and we will continue to support them.
“Through our consistent support we have helped SME’s to improve competitiveness and encouraged wealth creation in the region.”
Finance Yorkshire provides seedcorn, loan and equity linked investments, ranging from £15,000 to £2m to help a range of SMEs to meet their funding requirements for growth and development.