Mark Jenkinson and Son, which has offices in Sheffield and Chesterfield, beat off competition from more than 20 other individual auctioneers across the region with sales totalling £7.5m more than the auctioneer ranked in second place.
The research by Essential Information Group (EIG), which analyses UK-wide auction data, found Mark Jenkinson and Son also sold the most lots between January and December last year.
In total £5.25m of property was sold during the record auction for the firm, while 91% of its lots were sold.
Adrian Little, head of the auction department at Mark Jenkinson and Son, said: “To have the sold most lots or generated the highest sales would each be wonderful accomplishments in themselves, so to be ranked so highly in both categories is doubly pleasing and a really significant achievement for us.
“EIG analyse all auction data and are held in high regard amongst the country's auctioneers so we are thrilled to be recognised as the top ranking individual auctioneer for sales in Yorkshire and the Humber.”