Together they achieved sales of £4.1bn and profits of £912m in their last financial year, and collectively employ more than 12,000 people.
The highest-ranked company in the region is Bradford-based JCT600 at No 43 with sales of £1.1bn in 2015, up 12%. The Bradford-based car dealer group has 50 showrooms across northern England, and celebrated its 70th anniversary last month.
Northallerton-based R&R Ice Cream, at No 81 with sales of £720m, says it is the world’s third-largest ice cream maker. In April it announced a joint venture with Nestlé to sell ice cream and frozen food in more than 20 countries worldwide.
Doncaster’s housing specialist Keepmoat and Bradford’s Kelda Group also feature in the rankings.
Richard Flint, chief executive at Kelda Group, said: "We are pleased to see our ranking rise up to 46th in the Sunday Times HSBC Top Track 100 league table.
"But more important than our financial performance is our customer service performance so we want to let people know that over the next year we will be investing £318m to improve water and sewerage services, including drinking water quality and waste water treatment.
"Since privatisation in 1989, we have spent £20bn on maintaining and improving our water infrastructure such as sewers, reservoirs, pumping stations, water pipes, boreholes and water treatment works, which as a result means we now have truly world-class services to offer our five million customers."
Dan Howlett, head of corporate banking in the UK at HSBC, commented: “At a time of unprecedented uncertainty, we can take real comfort from the dynamic private companies across the UK as recognised by the Top Track 100 league table.
“Firms such as these are fundamental in helping to get Britain back to business during this post-Brexit period of uncertainty and the inevitable challenges ahead. These businesses are ambitious, agile and passionate about what they do and will play an important part in helping to drive the British economy forward, domestically and internationally."